|
KMID : 0545120070170061045
|
|
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 6 p.1045 ~ p.1048
|
|
|
Increase of CoQ10 Production Level by the Coexpression of Decaprenyl Diphosphate Synthase and 1-Deoxy-D-xylulose 5-Phosphate Synthase Isolated from Rhizobium radiobacter ATCC 4718 in Recombinant Escherichia coli
|
|
Seo Myung-Ji
Kim Soon-Ok
Im Eun-Mi
Nam Jung-Yeon
|
|
|
Abstract
|
|
|
|
Two genes, dps encoding decaprenyl diphosphate synthase and dxs encoding 1-deoxy-D-xylulose 5-phosphate synthase, were isolated from Rhizobium radiobacter ATCC 4718. DNA sequencing analysis of the dps and dxs genes revealed an open reading frame of 1,077 bp and 1,920 bp, respectively. The heterologous expression in Escherichia coli BL21(DE3) was carried out in order to identify their functions. Recombinant E. coli BL21(DE3) harboring the dps gene produced CoQ10 as well as CoQ8 and CoQ9, whereas E. coli harboring only the dxs gene produced more CoQ8 compared with the wildtype E. coli. Additionally, the coexpression of dps and dxs genes in E. coli was carried out. The recombinant E. coli harboring only the dps gene produced 0.21±0.04 mg/l of CoQ10, whereas the coexpressed E. coli with dps and dxs genes produced 0.37±0.07 mg/l of CoQ10. HPLC analysis also showed that the CoQ10 fraction (100% of the total CoQs distribution) was increased from 15.86±0.66% (only dps) to 29.78±1.80% (dps and dxs).
|
|
|
KEYWORD
|
|
|
Coenzyme Q10, decaprenyl diphosphate synthase, 1-deoxy-D-xylulose 5-phosphate synthase, Rhizobium radiobacter
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|